metal chelate affinity chromatography meaning in Chinese
金属螯合亲和层析
Examples
- The expressed product is soluble , mw 18kd , which was purified by ni + metal chelate affinity chromatography
表达产物为可溶性蛋白，分子量约为18kd ，通过ni ~ +离子亲和层析一次性纯化。 - The yeast recombinant expression vector pplc3 . 5k - ndrg2 was constructed , then the ndrg2 protein was expressed in soluable form in pichia . after purification by metal chelate affinity chromatography , we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future
为研究ndrgz的结构与功能，构建了ndrgz的酵母融合表达质粒，并在比赤酵母中得到可溶形式的表达；利用金属螫合亲合层析纯化出了6his融合的ndrgz蛋白。